Chromosomal Mutagenesis (Methods in Molecular Biology)

This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique and one that is available to any molecular biology laboratory, and this collection provides detailed protocols, case-studies, and reviews from thought-leaders in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and fully updated, Chromosomal Mutagenesis, Second Edition aims to help speed scientific discovery and aid in the next advances in the field. 1. Genome Editing by Targeted Chromosomal Mutagenesis             Dana Carroll   2. piggyBac Transposon-Based Insertional Mutagenesis in Mouse Haploid Embryonic Stem Cells             Stephen J. Pettitt, E-Pien Tan, and Kosuke Yusa   3. Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy             Jonathan M. Geisinger and Michele P. Calos   4. Genome Mutagenesis Using Synthetic Triplex-Forming Molecules and Recombinagenic DNA             Faisal Reza and Peter M. Glazer   5. Genome Engineering Using Adeno-Associated Virus (AAV)             Rob Howes and Christine Schofield   6. Engineering of Customized Meganucleases Via In Vitro Compartmentalization and In Cellulo Optimization             Ryo Takeuchi, Michael Choi, and Barry L. Stoddard   7. Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform             Tomas Cermak, Colby G. Starker, and Daniel F. Voytas   8. Ligation-Independent Cloning (LIC) Assembly of TALENs             Jonathan L. Schmid-Burgk, Tobias Schmidt, and Veit Hornung   9. Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications             Sandrine Boissel and Andrew M. Scharenberg   10. Genome Engineering Using CRISPR-Cas9 System             Le Cong and Feng Zhang   11. Donor Plasmid Design for Codon and Single Base Genome Editing Using Zinc Finger Nucleases             Shondra M. Pruett-Miller and Gregory D. Davis   12. Endogenous Gene Tagging with Fluorescent Proteins             John Fetter, Andrey Samsonov, Nathan Zenser, Fan Zhang, Hongyi Zhang, and Dmitry Malkov   13. Silencing Long Non-Coding RNAs with Genome-Editing Tools             Tony Gutschner   14. Gene Editing Using ssODNs with Engineered Endonucleases             Fuqiang Chen, Shondra M. Pruett-Miller, and Gregory D. Davis   15. Genome Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases             Kunitoshi Chiba and Dirk Hockemeyer   16. Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Cells             Matthew Porteus   17. Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models             Victoria M. Bedell and Stephen C. Ekker   18. Creating Knockout and Knockin Rodents Using Engineered Endonucleases Via Direct Embryo Injection             Takehito Kaneko and Tomoji Mashimo   19. Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains             Takehito Kaneko