Planar Cell Polarity: Methods and Protocols (Methods in Molecular Biology)

Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states.  In Planar Cell Polarity: Methods and Protocols, expert researchers in the field present a number of detailed and well-designed protocols used successfully in their labs around the world.  As a volume in the highly successful Methods in Molecular Biology(TM) series, the chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Timely and authoritative, Planar Cell Polarity: Methods and Protocols serves as a valuable reference for both novices and experts in this dynamic and versatile area of study. 1. Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Locations             Daryl J.V. David, Melanie A. McGill, R.F. Andrew McKinley, and Tony J.C. Harris   2. Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell             Bertrand Jauffred and Yohanns Bellaiche   3. Protein-Protein Interaction Techniques: Dissect PCP Signaling in Xenopus             Yingqun Wang   4. Cuticle Refraction Microscopy (CRM): A Rapid and Simple Method for Imaging Drosophila Wing Topography, an Alternative Readout of Wing Planar Cell Polarity             David Neff, Justin Hogan, and Simon Collier   5. Analysis of Cell Shape and Polarity during Zebrafish Gastrulation             Douglas C. Weiser and David Kimelman   6. Analyzing Planar Cell Polarity during Zebrafish Gastrulation             Jason R. Jessen   7. Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements during Xenopus Gastrulation             Gun-Hwa Kim, Edmond Changkyun Park, and Jin-Kwan Han   8. Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling             Hyun-Shik Lee, Sergei Y. Sokol, Sally A. Moody, and Ira O. Daar   9. Gene Loss-of-Function and Live Imaging in Chick Embryos             Anne C. Rios, Christophe Marcelle, and Olivier Serralbo   10. Activation and Function of Small GTPases Rho, Rac, and Cdc42 during Gastrulation             Courtney Mezzacappa, Yuko Komiya, and Raymond Habas   11. Convergent Extension Analysis in Mouse Whole Embryo Culture             Sophie E. Pryor, Valentina Massa, Dawn Savery, Nicholas D.E. Greene, and Andrew J. Copp   12. Analysis of PCP Defects in Mammalian Eye Lens             Yuki Sugiyama and John W. McAvoy   13. Examining Planar Cell Polarity (PCP) in the Mammalian Cochlea             Helen May-Simera and Matthew W. Kelley   14. Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells             Lisa Fujimura and Masahiko Hatano   15. The Planar Cell Polarity Pathway and Parietal Endoderm Cell Migration             Kristi LaMonica and Laura Grabel   16. Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells             Mitsuharu Endo, Michiru Nishita, and Yasuhiro Minami   17. Regulation of Focal Adhesion Dynamics by Wnt5a Signaling             Shinji Matsumoto and Akira Kikuchi   18. The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity             Makoto Matsuyama and Akihiko Shimono   19. Assessing PCP in the Cochlea of Mammalian Ciliopathy Models             Daniel J. Jagger and Andrew Forge   20. Morphometric Analysis of Centrosome Position in Tissues             Hester Happ, Emile de Heer, and Dorien J.M. Peters