1. Generation of Cerebral Cortical Neurons from Human Pluripotent Stem Cells in 3D Culture Yuanwei Yan and Su-Chun Zhang 2. Generation of Homogeneous Populations of Cortical Interneurons from Human Pluripotent Stem Cells Peiyan Ni, Lingyi Fan, Amy Zinski, and Sangmi Chung 3. Generation and Co-Culture of Cortical Glutamatergic and GABAergic Induced Neuronal Cells Jay English, Danny McSweeney, Fumiko Ribbe, Ethan Howell, and ChangHui Pak 4. Transcription Factor-Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells Yi Han Ng and Justyna A. Janas 5. Directed Differentiation of Human iPSCs into Microglia-Like Cells Using Defined Transcription Factors Shih-Wei Chen and Yu-Hui Wong 6. The Generation and Functional Characterization of Human Microglia-Like Cells Derived from iPS and Embryonic Stem Cells Mikael Lehoux, Kevin Connolly, Benedetta Assetta, and Yu-Wen Alvin Huang 7. Modeling Cellular Crosstalk of Neuroinflammation Axis by Tri-Cultures of iPSC-Derived Human Microglia, Astrocytes, and Neurons Kevin Connolly, Mikael Lehoux, Benedetta Assetta, and Yu-Wen Alvin Huang 8. Generation of Oligodendrocytes from Human Pluripotent and Embryonic Stem Cells Zoe Mattingly and Sundari Chetty 9. Characterizing the Neuron-Glial Interactions by the Co-Cultures of Human iPSC-Derived Oligodendroglia and Neurons Gabriella Vulakh and Xin Yang 10. Defined Differentiation of Human Pluripotent Stem Cells to Brain Microvascular Endothelial-Like Cells for Modeling the Blood-Brain Barrier Koji L. Foreman, Eric V. Shusta, and Sean P. Palecek 11. Modeling the Blood-Brain Barrier Using Human Induced Pluripotent Stem Cells Louise A. Mesentier-Louro, Natalie Suhy, Diede Broekaart, Michael Bula, Ana C. Pereira, and Joel W. Blanchard 12. A Three-Dimensional Primary Cortical Culture System Compatible with Transgenic Disease Models, Virally-Mediated Fluorescence, and Live Microscopy Sophie Brown, Elaina Atherton, and David A. Borton 13. Method to Generate Dorsal Forebrain Brain Organoids from Human Pluripotent Stem Cells Rebecca Sebastian, Narciso S. Pavon, Yoonjae Song, Karmen T. Diep, and ChangHui Pak 14. A 3D Bioengineered Neural Tissue Model Generated from Human iPSC-Derived Neural Precursor Cells Selene Lomoio and Giuseppina Tesco 15. FACS-Based Sequencing Approach to Evaluate Cell Type to Genotype Associations Using Cerebral Organoids Liam Murray, Meagan N. Olson, Nathaniel Barton, Pepper Dawes, Yingleong Chan, and Elaine T. Lim 16. Dynamic Measurement of Endosome-Lysosome Fusion in Neurons Using High-Content Imaging Qing Ouyang, Michael Schmidt, and Eric M. Morrow 17. Live-Imaging Detection of Multivesicular Body-Plasma Membrane Fusion and Exosome Release in Cultured Primary Neurons Matthew F. Pescosolido, Qing Ouyang, Judy S. Liu, and Eric M. Morrow 18. Assays of Monitoring and Measuring Autophagic Flux for iPSC-Derived Human Neurons and Other Brain Cell Types Ryan O’Rourke, Guzide Ayse Erdemir, and Yu-Wen Alvin Huang 19. Measuring Neuronal Network Activity Using Human Induced Neuronal Cells Danny McSweeney, Jay English, Ethan Howell, Fumiko Ribbe, and ChangHui Pak 20. A Simple Ca2+-Imaging Approach of Network-Activity Analyses for Human Neurons Zijun Sun 21. Whole Cell Patch Clamp Electrophysiology in Human Neuronal Cells Rafael Gabriel III, Andrew J. Boreland, and Zhiping P. Pang 22. Assaying Chemical Long-Term Potentiation in Human iPSC-Derived Neuronal Networks Deborah Pr, Alexander T. Wooten, Haowen Zhou, Ashley Neil, and Anne G. Bang
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